In vitro pO2 calibration

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Coefficient of variation (CV) values for each in vitro sample
% O2 CV
0 0.04
95 0.08
159.6 0.06
190 0.05
380 0.08
570 0.02
760 0.02 

## 
## Call:
## lm(formula = full_calib_data$mean_R1 ~ full_calib_data$O2_partial_pressure)
## 
## Residuals:
##         1         2         3         4         5         6         7 
##  0.000469  0.111032  0.010414 -0.172834  0.022644  0.014938  0.013338 
## 
## Coefficients:
##                                      Estimate Std. Error t value Pr(>|t|)
## (Intercept)                         0.4251265  0.0551145   7.714 0.000585
## full_calib_data$O2_partial_pressure 0.0021241  0.0001379  15.399  2.1e-05
##                                        
## (Intercept)                         ***
## full_calib_data$O2_partial_pressure ***
## ---
## Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
## 
## Residual standard error: 0.09298 on 5 degrees of freedom
## Multiple R-squared:  0.9793, Adjusted R-squared:  0.9752 
## F-statistic: 237.1 on 1 and 5 DF,  p-value: 2.096e-05

## [1] "R1=  0.425126474920255  +  0.00212411869128834 *pO2"
## [1] "95 % CI of regression model"
##                                           2.5 %      97.5 %
## (Intercept)                         0.283450025 0.566802925
## full_calib_data$O2_partial_pressure 0.001769531 0.002478706

In vivo data quality control

Mouse Signal All 3 gas challenges? R1 fitting day 0* R1 fitting day 1* R1 fitting day 7* R1 fitting day 28*
M02 Good Y Y - - -
M03 Good Y Y Y Y Y
M05 Good Y Y N N Y
M08 Good Y Y Y Y Y
M09 Sufficient N Y N N Y
M10 Sufficient N Y N N Y
M15 Sufficient Y N N N N
M01 Low N X - - -
M06 Low N X X X X
M11 Low N X X X -
M13 Low N X X X X
M16 Low N X X - -
M04 Absent N X - - -
M07 Absent N X X X -
M12 Absent N X X X -
M14 Absent N X - - -
M17 Absent N X X - -

Note: * Y refers to successful R1 fitting, N to unsuccessful fitting, X to ineligibility for fitting (because of low signal), and - to absence of FAIR-EPI data (i.e. mouse not scanned).

In vivo optimization (gas challenges)

In vivo pO2 changes over time